Coding

Part:BBa_K3868050:Design

Designed by: Yan Xu   Group: iGEM21_NNU-China   (2021-10-15)


sgRNA-8G


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 139
    Illegal XbaI site found at 145
    Illegal SpeI site found at 30
    Illegal PstI site found at 157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 139
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
    Illegal PstI site found at 157
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 139
    Illegal BglII site found at 169
    Illegal XhoI site found at 193
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 139
    Illegal XbaI site found at 145
    Illegal SpeI site found at 30
    Illegal PstI site found at 157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 139
    Illegal XbaI site found at 145
    Illegal SpeI site found at 30
    Illegal PstI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Gene from S. pyogenes for the pTarget plasmid of the Crispr-Cas9 system

References